Question 1

Two different nucleic acids can _____ by annealing at their complementary sites.

Accepted Answer

A)  form a peptide bond
B)  covalently bond
C)  ligate
D)  hybridizeE) None of the above
F) A) and C) D

Question 2

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 3

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work.Which of the following is a possible explanation for this negative result?

Accepted Answer

A)  You forgot to label the probe sequence with a reporter molecule.
B)  You forgot to add the mRNA to the DNA probe sample.
C)  You forgot to digest the probe with restriction endonucleases.
D)  You forgot to incubate the test at the 75oC temperature required for hybridization.E) All of the above
F) C) and D)

Question 4

If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ____ fragments.

Accepted Answer

A)  12
B)  24
C)  27
D)  48
E)  81F) B) and E)
G) A) and D)

Question 5

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)  principal sources of restriction endonucleases.
B)  used as cloning vectors.
C)  genetically engineered bacteria.
D)  sources of heat-stable DNA polymerases.E) B) and D)
F) A) and B)

Question 6

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Accepted Answer

A)  Whole genome sequencing
B)  Polymerase chain reaction
C)  DNA probe analysis
D)  Microarray analysisE) B) and D)
F) All of the above

Question 7

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 8

SNPs are derived from ______.

Accepted Answer

A)  genetic engineering
B)  frameshift mutations
C)  gene therapy
D)  point mutationsE) C) and D)
F) B) and D)

Question 9

DNA fragments can be separated in gel electrophoresis because

Accepted Answer

A)  phosphate groups have a net negative charge.
B)  nitrogenous bases have a net negative charge.
C)  nitrogenous bases have a net positive charge.
D)  phosphate groups have a net positive charge.E) B) and D)
F) A) and D)

Question 10

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA
D)  gel electrophoresis
E)  gene probesF) A) and B)
G) C) and D)

Question 11

A proteome is an organism's full array of expressed proteins, and proteomics is the study of those proteins and the functions they mediate.

Accepted Answer

A) True 
 B)False

Question 12

Before the advent of biotechnology, diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Accepted Answer

A)  gel electrophoresis
B)  gene therapy
C)  PCR
D)  recombinant DNA technologyE) B) and D)
F) A) and B)

Question 13

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called ______.

Accepted Answer

A)  cloning
B)  gene therapy
C)  antisense therapy
D)  DNA fingerprintingE) B) and D)
F) B) and C)

Question 14

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA
D)  gel electrophoresis
E)  gene probesF) None of the above
G) B) and D)

Question 15

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Accepted Answer

A)  Probes can be applied to intact cells.
B)  Probes can locate genes on chromosomes.
C)  It can be used to identify unknown bacteria without culturing.
D)  It uses electrophoresis to separate the DNA.
E)  It can be used to detect RNA in cells.F) D) and E)
G) B) and C) D

Question 16

A technique that separates a readable pattern of DNA fragments is ______.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA
D)  gel electrophoresis
E)  gene probesF) A) and D)
G) D) and E)

Question 17

Transgenic animals

Accepted Answer

A)  can be engineered to become factories for manufacturing proteins.
B)  are often obtained from germline engineering.
C)  will pass the genes on to their offspring.
D)  commonly include mice.
E)  All of the choices are correct.F) A) and D)
G) D) and E)

Question 18

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Accepted Answer

A)  palindromes
B)  reverse transcriptases
C)  restriction endonucleases
D)  ligases
E)  DNA polymerasesF) None of the above
G) C) and D)

Question 19

In the formation of recombinant DNA, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Accepted Answer

A)  DNA polymerase I
B)  DNA polymerase II
C)  DNA helicase
D)  DNA ligase
E)  PrimaseF) C) and D)
G) B) and D)

Question 20

Viruses are often used as cloning hosts in recombinant DNA methods.

Accepted Answer

A) True 
 B)False  False

Exam 10: Genetic Engineering and Recombinant Dna

Two different nucleic acids can _____ by annealing at their complementary sites.

Correct Answer
verified
D

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Correct Answer
verified

Correct Answer
verified

If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ____ fragments.

Correct Answer
verified

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct Answer
verified

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct Answer
verified

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Correct Answer
verified

SNPs are derived from ______.

Correct Answer
verified

DNA fragments can be separated in gel electrophoresis because

Correct Answer
verified

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Correct Answer
verified

A proteome is an organism's full array of expressed proteins, and proteomics is the study of those proteins and the functions they mediate.

Correct Answer
verified

Before the advent of biotechnology, diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Correct Answer
verified

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called ______.

Correct Answer
verified

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Correct Answer
verified

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Correct Answer
verified
D

A technique that separates a readable pattern of DNA fragments is ______.

Correct Answer
verified

Transgenic animals

Correct Answer
verified

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Correct Answer
verified

In the formation of recombinant DNA, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Correct Answer
verified

Viruses are often used as cloning hosts in recombinant DNA methods.

Correct Answer
verified
False

Exam 10: Genetic Engineering and Recombinant Dna

Two different nucleic acids can _____ by annealing at their complementary sites.

Correct AnswerverifiedD

After three replication cycles in PCR, there will be a total of three double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ____ fragments.

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Correct AnswerverifiedShow Answer

The process of introducing a needed, normal gene into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

SNPs are derived from ______.

Correct AnswerverifiedShow Answer

DNA fragments can be separated in gel electrophoresis because

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Correct AnswerverifiedShow Answer

A proteome is an organism's full array of expressed proteins, and proteomics is the study of those proteins and the functions they mediate.

Correct AnswerverifiedShow Answer

Before the advent of biotechnology, diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Correct AnswerverifiedShow Answer

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called ______.

Correct AnswerverifiedShow Answer

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Correct AnswerverifiedShow Answer

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Correct AnswerverifiedD

A technique that separates a readable pattern of DNA fragments is ______.

Correct AnswerverifiedShow Answer

Transgenic animals

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Correct AnswerverifiedShow Answer

In the formation of recombinant DNA, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Correct AnswerverifiedShow Answer

Viruses are often used as cloning hosts in recombinant DNA methods.

Correct AnswerverifiedFalse

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
D

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
False